Three-dimensional Fluorescence Microscopy by Optical Scanning Holography

In optical sectioning microscopy (OSM), 3-D information is collected by recording a series of 2-D images at various focal planes throughout the 3-D specimen.1 However, during the recording of 2-D images, it is critical that exact longitudinal spacing between adjacent 2-D images and the precise registration of these image slides are accurately controlled. Scanning confocal microscopy (SCM) is a radically new microscope design.

Access to the full text of this article is restricted. In order to view this article please log in.


Add a Comment

comments powered by Disqus