Objective for Fluorescence Microscopy

One of the main problems in microscopy is that many objects of interest are invisible under ordinary lighting conditions. Most materials that are opaque and therefore easily seen in bulk form, become nearly transparent phase objects when reduced to sub-micron thickness. One technique for enhancing the contrast of microscopic subjects is to illuminate them with UV light and then observe the emitted fluorescent radiation.

Become a member of OSA or log in to view the full text of this article.

OSA Members get the full text of Optics & Photonics News, plus a variety of other member benefits.

Add a Comment

comments powered by Disqus