This is an agile volume that brings the reader from the very basic fundamentals of microscopy to modern techniques such as advanced wide-field microscopy, confocal microscopy, fluorescence-lifetime imaging, light-sheet and selective-plane microscopy, and multiphoton fluorescence. The book includes a four-chapter, cutting-edge exposition of new research advances in microscopy fields like harmonic, Raman, digital holographic and super-resolution. Two final chapters cover practical issues like how to obtain the most from the data and selection criteria for optical microscopy.

A distinctive feature of the volume is the guide it offers for making optimal use of the instrument and developing research protocols.

Review by Silvano Donati, University of Pavia, Italy

The opinions expressed in the book review section are those of the reviewer and do not necessarily reflect those of OPN or OSA.