The editors and contributors have produced an outstanding book suitable for people with interests in the biomedical applications of super-resolution microscopy. Several factors resulted in this recommendation. The comprehensive chapter on deconvolution is unique in the microscopy literature as it clearly states the assumptions of each technique as well as the practical limitations. The chapter on structured-light microscopy is another gem, with its exposition of theory, instrumentation and limitations.
The warning of artifacts in the specimen preparation, the image acquisition, as well as image analysis and interpretation, permeate the book’s content. Similarly, the cautions on the effects of the high light intensity associated with stimulated emission depletion (STED) microscopy for deep tissue and live-cell imaging are appropriate. The chapters that discuss correlative microscopy are critical for users of super-resolution imaging. The figures are all high quality and supplement the text.

Review by Barry R. Masters, Fellow of AAAS, OSA and SPIE.

The opinions expressed in the book review section are those of the reviewer and do not necessarily reflect those of OPN or OSA.