This book describes several modern techniques based on fluorescence microscopy with applications in cell biology. Its strength is the detailed comparisons of the methods for enhancing resolution and contrast; for example, tissue clearing techniques and commercial super-resolution microscopes. The editors provide good coverage of protocols, algorithms and correlative microscopy. However, the level of physical explanation is uneven. Certain chapters contain clear explanations of the physics and mathematics involved, but others provide only weak descriptions of the physics fundamental to each microscopic technique. The intended audience is individuals who use commercial microscopes to investigate biological problems. There are color figures, critical references and an index, which help the reader understand the text.

Review by Barry R. Masters, Fellow of AAAS, OSA and SPIE. 

The opinions expressed in the book review section are those of the reviewer and do not necessarily reflect those of OPN or OSA.