Lasers And Optics In Flow Cytometry
John A. Steinkamp
The application of flow cytometric (FCM) instruments for automated analysis and separation of cells and organelles has provided new dimensions to biomedical research and clinical medicine by making it possible to analyze and isolate cells and organelles, e.g., chromosomes, with particular physical, biochemical, and immunological properties. This technology represents an exciting departure from conventional microscope-based cytophotometric procedures that require up to several hours to measure and process several hundred cells. In FCM systems, the uniqueness is that multiple measurements are made on a cell-by-cell basis at rates up to 3 X 105/min., statistical precision is high, and subpopulations are detected and separated from heterogeneous mixtures for identification and/or for functional and biochemical analyses.
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